Atletico Madrid and Inter Milan decided to drop out of the Super League on Wednesday.
The moves came a day after the six clubs in the Premier League withdrew from the controversial breakaway race, leaving only four clubs still involved.
Atletico said the decision was made after a meeting of the board of directors on Wednesday.
The Spanish club said it “decided to officially communicate the Super League, and the other founding clubs decided not to formalize their participation in the project.”
Inter said the club is committed to providing the best football experience for fans because “innovation and inclusion have been part of our DNA since our inception.”
“Our commitment to all stakeholders to improve the football industry will never change,” said the Italian club.
Spanish clubs Real Madrid and Barcelona also competed in the Super League, along with Italian clubs Milan and Juventus.